Design

The foundation of our circuit

Our circuit was designed to utilize specific parts so that when the E-coli cells containing this gene circuit are exposed to arsenic, the Green Florescent Protein (GFP) is produced making the ticket glow under a blue light. 

PHASE 1

  • Test efficacy of the circuit in vivo 
  • Grow transformed e.coli with plasma circuit in Arsenic laced media   
  • Serial dilution of arsenic and arsenic to find the lowest activation amount of the circuit in vivo

PHASE 2

  • Test the efficacy of the plasma circuit in S.I.T.U.   
  • Perform above experiments with prepared plasma circuit   
  • Titrate both circuit and trigger to find optimal mixture   
  • Standardize reactions    
  • Test on paper matrix

PHASE 3

  • Test efficacy of freeze drying circuit on paper for distribution and storage purposes.    


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